2PGD
THE STRUCTURE OF 6-PHOSPHOGLUCONATE DEHYDROGENASE REFINED AT 2 ANGSTROMS RESOLUTION
Replaces: 1PGDExperimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.740, 148.400, 102.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.340 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 5-10 (mg/ml) | |
| 2 | 1 | drop | 5'-ADP | 30 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 40 (%sat) | |
| 4 | 1 | reservoir | ammonium sulfate | 52-54 (%sat) |
