2PG4

Crystal structure of a putative dna binding protein (ape_0880a) from aeropyrum pernix k1 at 2.21 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97929
Spacegroup name	P 4 21 2
Unit cell lengths	109.840, 109.840, 36.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.430 - 2.210
R-factor	0.20083
Rwork	0.198
R-free	0.25754
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.650
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.437		2.270
High resolution limit [Å]	2.210	3.740	2.210
Rmerge	0.062	0.046	0.464
Number of reflections	11747
<I/σ(I)>	11.29	22.8	2.6
Completeness [%]	95.4	99.8	63.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	NANODROP, 1.0M LiCl, 10.0% PEG 6000, 0.1M Citrate pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K