2PFV
S. cerevisiae Exo70 with additional residues to 2.1 Angrstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-09-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.541, 60.073, 222.733 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.840 - 2.100 |
| Rwork | 0.237 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.086 | 0.357 |
| Number of reflections | 64253 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 95.4 | 74 |
| Redundancy | 6.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 278.15 | 20.5% PEG 300, 0.2M lithium chloride, 0.1M glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 278.15K |
