2PFT

The Crystal Structure of Mouse Exo70 Reveals Unique Features of the Mammalian Exocyst

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	77
Detector technology	CCD
Collection date	2006-07-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97926,0.97942,0.95660
Spacegroup name	P 32 2 1
Unit cell lengths	61.520, 61.520, 294.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.840 - 2.250
Rwork	0.235
R-free	0.28500
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.840	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.054	0.416
Number of reflections	30334
<I/σ(I)>	15.8
Completeness [%]	93.7	77.7
Redundancy	4.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	Precipitant in drop: 10% ethylene glycol, 5% PEG 8000, 0.01M magnesium chloride, 0.1M HEPES Precipitant in well: 10% ethylene glycol, 8% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	Precipitant in drop: 10% ethylene glycol, 5% PEG 8000, 0.01M magnesium chloride, 0.1M HEPES Precipitant in well: 10% ethylene glycol, 8% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	Precipitant in drop: 10% ethylene glycol, 5% PEG 8000, 0.01M magnesium chloride, 0.1M HEPES Precipitant in well: 10% ethylene glycol, 8% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K