2PFI
Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.283, 83.421, 59.888 |
| Unit cell angles | 90.00, 92.48, 90.00 |
Refinement procedure
| Resolution | 14.890 - 1.600 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.21800 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.278 |
| Number of reflections | 43653 | |
| <I/σ(I)> | 26.9 | 2.8 |
| Completeness [%] | 96.9 | 79 |
| Redundancy | 3.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 20% PEG 4000, 100mM KI, 60mM Tris-HCl, 150mM NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
