2PFC

Structure of Mycobacterium tuberculosis Rv0098

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	121
Detector technology	CCD
Detector	MAR CCD 165 mm
Spacegroup name	I 21 3
Unit cell lengths	101.767, 101.767, 101.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.560 - 2.300
R-factor	0.2368
Rwork	0.235
R-free	0.27311
RMSD bond length	0.018
RMSD bond angle	2.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.560
High resolution limit [Å]	2.190
Rmerge	0.081
Number of reflections	7549
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1 M Hepes, 15% isopropanol, 0.2M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K