2PFC
Structure of Mycobacterium tuberculosis Rv0098
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 121 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Spacegroup name | I 21 3 |
Unit cell lengths | 101.767, 101.767, 101.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.560 - 2.300 |
R-factor | 0.2368 |
Rwork | 0.235 |
R-free | 0.27311 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.131 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.560 |
High resolution limit [Å] | 2.190 |
Rmerge | 0.081 |
Number of reflections | 7549 |
Redundancy | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes, 15% isopropanol, 0.2M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |