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2PF5

Crystal Structure of the Human TSG-6 Link Module

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-2
Synchrotron siteESRF
BeamlineID14-2
Temperature [K]100
Detector technologyCCD
Collection date2005-08-27
DetectorADSC QUANTUM 4
Wavelength(s)0.933
Spacegroup nameC 1 2 1
Unit cell lengths134.816, 75.870, 90.674
Unit cell angles90.00, 103.42, 90.00
Refinement procedure
Resolution88.040 - 1.900
R-factor0.19596
Rwork0.194
R-free0.22588
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1UUH - crystal structure of CD44
RMSD bond length0.016
RMSD bond angle1.660
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareDM
Refinement softwareREFMAC (5.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]88.0402.000
High resolution limit [Å]1.9001.900
Rmerge0.0710.410
Number of reflections69439
<I/σ(I)>6.351.51
Completeness [%]99.198.62
Redundancy4.083.98
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.5293A 2.5 ul drop formed from equal amounts of protein solution (7 mg/ml solution in approximately 25mM TrisHCl and 75mM NaCl) and well solution was equilibrated against 1 ml well solution (2 M Ammonium Sulphate, 2 % v/v PEG 400 and 0.1M Sodium Hepes (pH 7.5)), VAPOR DIFFUSION, SITTING DROP, temperature 293K

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