2PEO
Crystal structure of RbcX from Anabaena CA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07200 |
| Spacegroup name | P 65 |
| Unit cell lengths | 69.021, 69.021, 113.805 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.244 |
| Rwork | 0.241 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pen |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.448 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.774 | 59.760 | 2.530 |
| High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
| Rmerge | 0.078 | 0.027 | 0.444 |
| Total number of observations | 1427 | 4442 | |
| Number of reflections | 11805 | ||
| <I/σ(I)> | 8.3 | 19.3 | 1.7 |
| Completeness [%] | 98.1 | 96.6 | 88.2 |
| Redundancy | 3.6 | 3.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 1.0 M Ammonium phosphate, 0.1 M Sodium citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
