2PEN

Crystal structure of RbcX, crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9789
Spacegroup name	P 41 21 2
Unit cell lengths	93.335, 93.335, 411.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.242
Rwork	0.240
R-free	0.26200
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	1.406
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE (2.01)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	411.990	91.030	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge	0.080	0.036	0.641
Total number of observations		20522	98241
Number of reflections	46362
<I/σ(I)>	6.5	14.8	1.2
Completeness [%]	100.0	99.2	100
Redundancy	14.5	12.1	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.30-1.55 M Sodium acetate, 0.1 M HEPES-NaOH pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.30-1.55 M Sodium acetate, 0.1 M HEPES-NaOH pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K