2PE8
Crystal structure of the UHM domain of human SPF45 (free form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.976 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 63.900, 90.200, 99.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.580 - 2.000 |
| R-factor | 0.23204 |
| Rwork | 0.231 |
| R-free | 0.24348 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UHM domain homology model based on PDB-ID 1o0p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.236 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.474 |
| Number of reflections | 9819 | |
| <I/σ(I)> | 20.13 | 4.67 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.57 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 22% PEG 3,350, 0.2 M MgCl2, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
