2PDR

1.7 Angstrom Crystal Structure of the Photo-excited Blue-light Photoreceptor Vivid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.10
Spacegroup name	P 1 21 1
Unit cell lengths	62.970, 81.130, 64.730
Unit cell angles	90.00, 89.94, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.23
Rwork	0.226
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pd7
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.102	0.431
Number of reflections	59234
<I/σ(I)>	13.31	1.91
Completeness [%]	82.9	63.6
Redundancy	2.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	30% PEG 5000 MME, 0.1M tri-sodium citrate, 0.1M ammonium acetate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	30% PEG 5000 MME, 0.1M tri-sodium citrate, 0.1M ammonium acetate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	30% PEG 5000 MME, 0.1M tri-sodium citrate, 0.1M ammonium acetate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	30% PEG 5000 MME, 0.1M tri-sodium citrate, 0.1M ammonium acetate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K