2PD2

Crystal structure of (ST0148) conserved hypothetical from Sulfolobus Tokodaii Strain7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-19
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	112.272, 112.272, 112.272
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.500 - 2.060
R-factor	0.186
Rwork	0.186
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l1s
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.120
High resolution limit [Å]	2.060	2.060
Rmerge	0.060	0.200
Number of reflections	14722
Completeness [%]	98.3	82.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	OIL-BATCH	8.5	293	0.2M Magnesium chloride hexahydrate, 0.1M Tris-HCl, 30% PEG 4000, pH 8.5, OIL-BATCH, temperature 293K