2PCS
Crystal structure of conserved protein from Geobacillus kaustophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.977, 61.977, 106.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.242 |
| Rwork | 0.239 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.937 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.912 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.114 | 0.642 |
| Number of reflections | 8605 | |
| <I/σ(I)> | 29 | 3.5 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 24.5 | 23.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 294 | 100mM Hepes pH 8.0, 2% PEG 400, 2M Ammonium sulfate, VAPOR DIFFUSION, temperature 294K |
