2PCP

ANTIBODY FAB COMPLEXED WITH PHENCYCLIDINE

Experimental procedure

Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-08
Detector	MACSCIENCE
Spacegroup name	P 1
Unit cell lengths	53.460, 67.720, 68.040
Unit cell angles	62.11, 84.66, 83.17

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.199 *
Rwork	0.196
R-free	0.28600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4FAB AND 1GIG
RMSD bond length	0.015 *
RMSD bond angle	2.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.400
High resolution limit [Å]	2.200	2.200
Rmerge	0.091	0.221
Total number of observations	69236 *
Number of reflections	33694
<I/σ(I)>	9.8	3.7
Completeness [%]	79.3	63.5
Redundancy	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	2.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)