2PCL
Crystal structure of ABC transporter with complex (aq_297) from aquifex aeolicus VF5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-15 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 104.177, 104.177, 135.046 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.800 - 1.700 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.24694 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f3o |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.031 | 0.249 |
| Number of reflections | 31017 | |
| Completeness [%] | 99.7 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.075M Sodium Acetate trihydrate, 1.5M Ammonium Sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
