2PBX
Vibrio cholerae HapR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-17 |
| Detector | ADSC |
| Wavelength(s) | 1.0093 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.785, 85.085, 110.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.470 - 2.200 |
| R-factor | 0.23298 |
| Rwork | 0.230 |
| R-free | 0.26260 |
| Structure solution method | RIPAS |
| RMSD bond length | 0.041 |
| RMSD bond angle | 3.189 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.200 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.059 | 0.206 |
| Number of reflections | 21439 | |
| <I/σ(I)> | 17.68 | 5.69 |
| Completeness [%] | 98.3 | 99.1 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 0.2 M HEPES pH 7.5, 12% (wt/vol) polyethylene glycol 8000, and 5% (vol/vol) ethylene glycol, VAPOR DIFFUSION, HANGING DROP | ||
| 1 | VAPOR DIFFUSION, HANGING DROP | 0.2 M HEPES pH 7.5, 12% (wt/vol) polyethylene glycol 8000, and 5% (vol/vol) ethylene glycol, VAPOR DIFFUSION, HANGING DROP |
