2PBE
Crystal structure of an aminoglycoside 6-adenyltransferase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 106.562, 106.560, 75.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.630 - 2.650 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.28400 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.117 | 0.278 |
| Number of reflections | 12063 | |
| <I/σ(I)> | 9.8 | 5.1 |
| Completeness [%] | 91.7 | 50.9 |
| Redundancy | 20.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 20% PEG 4000, 10% Isopropanol, 0.1 M Na-HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
