2PBD
Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90020 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.094, 56.589, 75.227 |
| Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
| Resolution | 31.770 - 1.501 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pav |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.695 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.071 | 0.293 |
| Number of reflections | 75393 | |
| <I/σ(I)> | 41.9 | 9 |
| Completeness [%] | 97.5 | 98.3 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277.15 | 150mM DL-malic acid pH 7.0, 18% PEG 3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
