2PAX
THE CATALYTIC FRAGMENT OF POLY(ADP-RIBOSE) POLYMERASE COMPLEXED WITH 4-AMINO-1,8-NAPHTHALIMIDE
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-11 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.230, 64.530, 96.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.100 - 2.400 |
R-factor | 0.167 |
Rwork | 0.167 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1pax |
RMSD bond length | 0.010 |
RMSD bond angle | 23.620 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.100 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.073 | 0.232 |
Total number of observations | 49815 * | |
Number of reflections | 13590 | |
<I/σ(I)> | 13.8 | 7.3 |
Completeness [%] | 95.0 * | 73 * |
Redundancy | 3.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | PROTEIN WAS CRYSTALLIZED FROM 12% PEG 600, 6% ISOPROPANOL, 100 MM TRIS, PH 8.5 AND 0.03MM 4-AMINO-1,8-NAPHTALIMIDE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | beta-octylglucoside | 0.1 (%(w/v)) | |
3 | 1 | drop | Ap4A | 5 (mM) | |
4 | 1 | reservoir | PEG4000 | 17 (%(w/v)) | |
5 | 1 | reservoir | 2-propanol | 8 (%(v/v)) | |
6 | 1 | reservoir | Tris-HCl | 70 (mM) |