2PAG

Crystal structure of protein PSPTO_5518 from Pseudomonas syringae pv. tomato

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	47.424, 47.424, 122.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.710 - 1.600
R-factor	0.233
Rwork	0.233
R-free	0.22200
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.600
Rmerge	0.044
Number of reflections	18902
Completeness [%]	98.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	30% PEG 400, 0.1 M Hepes, 0.2 M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K