2PA4
Crystal structure of UDP-glucose pyrophosphorylase from Corynebacteria glutamicum in complex with magnesium and UDP-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99848 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.400, 47.600, 161.500 |
| Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.24300 |
| Structure solution method | SIR |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.198 |
| Number of reflections | 78712 | |
| <I/σ(I)> | 33.7 | 4.5 |
| Completeness [%] | 88.8 | 83.9 |
| Redundancy | 6.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 23% PEG-3400, 200 mM MgCl2, 200 mM NaCl, 10 mM UDP-glucose, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
