2P9S

Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	111.020, 128.694, 201.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.680
R-factor	0.224
Rwork	0.222
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.780
High resolution limit [Å]	2.680	2.680
Number of reflections	80893
<I/σ(I)>	22.3	2.6
Completeness [%]	1.0	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K