2P9R
Human alpha2-macroglogulin is composed of multiple domains, as predicted by homology with complement component C3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.810, 68.810, 120.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.700 - 2.300 |
| Rwork | 0.244 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 2.300 |
| High resolution limit [Å] | 2.300 | 2.200 |
| Number of reflections | 13500 | |
| Completeness [%] | 98.6 | |
| Redundancy | 23 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | 11% PEG 3350, 200mM di-ammonium hydrogen citrate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
