2P9Q
Crystal Structure of Phosphoglycerate Kinase-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-16 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.044, 80.850, 92.900 |
Unit cell angles | 90.00, 94.63, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vjd |
RMSD bond length | 0.008 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.056 | 0.244 |
Number of reflections | 20415 | |
<I/σ(I)> | 14.3 | |
Completeness [%] | 95.9 | 92.8 |
Redundancy | 2.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG4000, 0.2 M sodium acetate, 0.1 M Tris-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.50 |