2P9M

Crystal structure of conserved hypothetical protein MJ0922 from Methanocaldococcus jannaschii DSM 2661

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-31
Detector	MARRESEARCH
Wavelength(s)	0.97240
Spacegroup name	P 21 21 21
Unit cell lengths	57.207, 94.688, 102.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.470 - 2.590
R-factor	0.22544
Rwork	0.222
R-free	0.29177
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.435
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SGXPRO
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.590	2.590
Number of reflections	17759
<I/σ(I)>	16.7	4.73
Completeness [%]	99.4	96.3
Redundancy	12.6	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH UNDER OIL	6.3	289	USING 1.0 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (27.49 mg/ml) AND SOLUTION CONTAINING 27.5% w/v PEG 4000, 0.1 M MES-SODIUM HYDROXIDE, pH 6.3, MICROBATCH UNDER OIL, temperature 289K