2P9M
Crystal structure of conserved hypothetical protein MJ0922 from Methanocaldococcus jannaschii DSM 2661
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97240 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.207, 94.688, 102.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.470 - 2.590 |
| R-factor | 0.22544 |
| Rwork | 0.222 |
| R-free | 0.29177 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.435 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SGXPRO |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 17759 | |
| <I/σ(I)> | 16.7 | 4.73 |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 12.6 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 6.3 | 289 | USING 1.0 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (27.49 mg/ml) AND SOLUTION CONTAINING 27.5% w/v PEG 4000, 0.1 M MES-SODIUM HYDROXIDE, pH 6.3, MICROBATCH UNDER OIL, temperature 289K |
