2P9H
High resolution structure of the Lactose Repressor bound to IPTG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.92 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.125, 77.125, 210.583 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.040 - 2.000 |
R-factor | 0.18776 |
Rwork | 0.185 |
R-free | 0.24552 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efa |
RMSD bond length | 0.017 |
RMSD bond angle | 1.710 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.136 | 0.264 |
Number of reflections | 36802 | |
<I/σ(I)> | 10.7 | 2.1 |
Completeness [%] | 83.6 | 37.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 281 | 1.4 M Sodium Acetate, 0.1 M Sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 281K |