2P9B
Crystal structure of putative prolidase from Bifidobacterium longum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.649, 63.045, 56.248 |
Unit cell angles | 90.00, 101.97, 90.00 |
Refinement procedure
Resolution | 40.270 - 1.700 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.21200 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 53450 | |
<I/σ(I)> | 20 | 2.5 |
Completeness [%] | 93.7 | 57.3 |
Redundancy | 7.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 12% PEG 6000, 0.1M Citric acid pH 5.0, 0.05 M Magnesium chloride, 5% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |