2P9B
Crystal structure of putative prolidase from Bifidobacterium longum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.649, 63.045, 56.248 |
| Unit cell angles | 90.00, 101.97, 90.00 |
Refinement procedure
| Resolution | 40.270 - 1.700 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 53450 | |
| <I/σ(I)> | 20 | 2.5 |
| Completeness [%] | 93.7 | 57.3 |
| Redundancy | 7.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 12% PEG 6000, 0.1M Citric acid pH 5.0, 0.05 M Magnesium chloride, 5% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
