2P8R
Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-21 |
| Wavelength(s) | 1.2830 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.225, 65.225, 136.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.230 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wild type |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.283 |
| Number of reflections | 17959 | |
| <I/σ(I)> | 34.8 | 6.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M Hepes, pH7, 14% PEG 4000, 0.22M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
