2P8R

Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-21
Wavelength(s)	1.2830
Spacegroup name	P 41 21 2
Unit cell lengths	65.225, 65.225, 136.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
Rwork	0.230
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	wild type
RMSD bond length	0.007
RMSD bond angle	1.381
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.064	0.283
Number of reflections	17959
<I/σ(I)>	34.8	6.9
Completeness [%]	99.9	100
Redundancy	7.6	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M Hepes, pH7, 14% PEG 4000, 0.22M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K