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2P8Q

Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X6A
Synchrotron siteNSLS
BeamlineX6A
Temperature [K]100
Detector technologyCCD
Collection date2005-03-20
DetectorADSC QUANTUM 210
Wavelength(s)0.97
Spacegroup nameP 1 21 1
Unit cell lengths64.752, 97.892, 84.286
Unit cell angles90.00, 90.86, 90.00
Refinement procedure
Resolution40.000 - 2.350
R-factor0.23
Rwork0.227
R-free0.25000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1qgk
RMSD bond length0.006
RMSD bond angle1.000
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0002.430
High resolution limit [Å]2.3502.350
Rmerge0.0820.476
Number of reflections44104
<I/σ(I)>20.12.66
Completeness [%]95.864.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP628522%PEG 8000, 0.05M Sodium Chloride, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K

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