2P8D
The Structure of the Dickerson Sequence with an Incorporated CeNA Residue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8414 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 24.915, 38.780, 66.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.760 - 1.900 |
| R-factor | 0.23313 |
| Rwork | 0.230 |
| R-free | 0.30752 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 355d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.803 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.457 |
| Number of reflections | 5476 | |
| <I/σ(I)> | 15.3 | 3 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 20mM potassium cacodylate buffer (pH=5.5), 10mM cobalt hexamine, 44mM potassium chloride, 6mM sodium chloride, 5%(v/v) 2-methyl-2,4-pentanediol (MPD), and 0.75mM ssDNA against a 35%(v/v) MPD stock solution, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | potassium cacodylate | ||
| 2 | 1 | 1 | cobalt hexamine | ||
| 3 | 1 | 1 | potassium chloride | ||
| 4 | 1 | 1 | sodium chloride | ||
| 5 | 1 | 1 | 2-methyl-2,4-pentanediol (MPD) | ||
| 6 | 1 | 2 | 2-methyl-2,4-pentanediol (MPD) |
