2P87
Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-08 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.063, 65.063, 135.552 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.226 |
R-free | 0.24900 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.408 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.051 | 0.274 |
Number of reflections | 23240 | |
<I/σ(I)> | 18.4 | 3.6 |
Completeness [%] | 92.6 | 96.2 |
Redundancy | 2.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M Hepes pH7, 14% PEK4K, 0.2M MgCl2, pH 7., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M Hepes pH7, 14% PEK4K, 0.2M MgCl2, pH 7., VAPOR DIFFUSION, HANGING DROP, temperature 298K |