2P7Z
Estrogen Related Receptor Gamma in complex with 4-hydroxy-tamoxifen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2003-10-15 |
Detector | BRUKER SMART 6000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.990, 63.990, 137.952 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.000 - 2.500 |
Rwork | 0.213 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ERRg BPA structure |
RMSD bond length | 0.008 |
RMSD bond angle | 1.158 |
Data reduction software | SAINT |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.090 | 0.300 |
Number of reflections | 9739 | |
<I/σ(I)> | 8.8 | 1.5 |
Redundancy | 7.37 | 7.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 22.6% polyethylene glycol 4000, 0.1M Tris pH 8.5 and 0.2M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |