2P7N

Crystal structure of the Pathogenicity island 1 effector protein from Chromobacterium violaceum. Northeast Structural Genomics Consortium (NESGC) target CvR69.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97912, 0.97897, 0.96862
Spacegroup name	I 2 2 2
Unit cell lengths	55.026, 66.981, 194.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.248
R-free	0.29800
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.089	0.038	0.665
Number of reflections	21495
<I/σ(I)>	12.3
Completeness [%]	99.9	99.3	100
Redundancy	7.5	7.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	150 mM Sodium acetate pH 4.6, 22% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	150 mM Sodium acetate pH 4.6, 22% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	150 mM Sodium acetate pH 4.6, 22% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K