2P7D
A Minimal, 'Hinged' Hairpin Ribozyme Construct Solved with Mimics of the Product Strands at 2.25 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.900, 93.900, 134.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.100 - 2.250 |
| R-factor | 0.2291 |
| Rwork | 0.229 |
| R-free | 0.23700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ZFR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.640 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 16622 | |
| <I/σ(I)> | 23.2 | 3.2 |
| Completeness [%] | 96.5 | 95.5 |
| Redundancy | 7.24 | 4.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 250 mM Li2SO4, 2.5 mM [Co(NH3)6]Cl3, 2 mM spermidine-HCl, 20 mM NAF, 50 mM ascorbic acid, 100 mM sodium cacodylate buffer and 18.5% PEG 2000 MME, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Li2SO4 | ||
| 10 | 1 | 2 | PEG 2000 MME | ||
| 2 | 1 | 1 | [Co(NH3)6]Cl3 | ||
| 3 | 1 | 1 | spermidine-HCl | ||
| 4 | 1 | 1 | NAF | ||
| 5 | 1 | 1 | ascorbic acid | ||
| 6 | 1 | 1 | sodium cacodylate | ||
| 7 | 1 | 1 | PEG 2000 MME | ||
| 8 | 1 | 2 | Li2SO4 | ||
| 9 | 1 | 2 | spermidine-HCl |
