2P7A
Crystal Structure of Estrogen Related Receptor g in complex with 3-methyl phenol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2003-12-18 |
Detector | BRUKER SMART 6000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 64.279, 64.279, 137.178 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.000 - 2.300 |
Rwork | 0.205 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ERRg BPA structure |
RMSD bond length | 1.976 |
RMSD bond angle | 1.257 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.103 | 2.390 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.118 | |
Number of reflections | 13237 | |
<I/σ(I)> | 5.9 | 1.55 |
Redundancy | 11.06 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 22.6% polyethylene glycol 4000, 0.1M Tris pH 8.5 and 0.2M Sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |