2P6R
Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.920 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 138.379, 138.379, 252.973 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
Rwork | 0.232 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.258 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.089 | 0.168 |
Number of reflections | 54172 | |
<I/σ(I)> | 16.93 | 8.97 |
Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0; 22% MPD; 0.1M MgAcetate; 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MES | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MgAcetate | ||
4 | 1 | 1 | Glycerol | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | MES | ||
7 | 1 | 2 | MPD | ||
8 | 1 | 2 | MgAcetate | ||
9 | 1 | 2 | Glycerol |