2P6N
Human DEAD-box RNA helicase DDX41, helicase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-29 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 68.013, 68.013, 305.604 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.720 - 2.600 |
R-factor | 0.24594 |
Rwork | 0.243 |
R-free | 0.29431 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i4i |
RMSD bond length | 0.016 |
RMSD bond angle | 1.602 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.103 | 0.450 |
Number of reflections | 13827 | |
<I/σ(I)> | 35.9 | 12.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 40 | 41.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 293 | 25% PEG 3350, 200mM Lithium sulfate, 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.50 |