2P6H
Crystal structure of hypothetical protein APE1520 from Aeropyrum pernix K1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 3 |
| Unit cell lengths | 73.023, 73.023, 49.433 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.370 - 1.950 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vph |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.047 | 0.205 |
| Number of reflections | 21386 | |
| <I/σ(I)> | 26.1 | 15 |
| Completeness [%] | 99.6 | 96.3 |
| Redundancy | 5.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | 0.1M HEPES, 1.5M Lithium Sulfate, pH7.5, MICROBATCH, temperature 291K |
