2P5X

Crystal structure of Maf domain of human N-acetylserotonin O-methyltransferase-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-01-28
Detector	RIGAKU RAXIS V
Wavelength(s)	1.54000
Spacegroup name	P 21 21 2
Unit cell lengths	84.802, 116.983, 51.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.070 - 2.000
R-factor	0.20077
Rwork	0.198
R-free	0.25309
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ex2
RMSD bond length	0.015
RMSD bond angle	1.429
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.070
High resolution limit [Å]	2.000
Rmerge	0.167
Number of reflections	35334
<I/σ(I)>	5.9
Completeness [%]	99.7
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	300	15% PEG 3350, 0.1 M Succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K