2P5V
Crystal Structure of Transcriptional Regulator NMB0573 from Neisseria Meningitidis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795, 0.9078 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.070, 148.480, 77.720 |
| Unit cell angles | 90.00, 106.41, 90.00 |
Refinement procedure
| Resolution | 29.590 - 1.990 |
| R-factor | 0.17933 |
| Rwork | 0.176 |
| R-free | 0.23785 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.424 |
| Number of reflections | 74733 | |
| <I/σ(I)> | 20.2 | 2.4 |
| Completeness [%] | 77.7 | 29.9 |
| Redundancy | 7.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 95 mM HEPES-Na, 190 mM Calcium Chloride, 26.6% PEG 400, 5% Glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
