2P5R
Crystal structure of the poplar glutathione peroxidase 5 in the oxidized form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.80630 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.580, 71.580, 117.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.450 |
| R-factor | 0.218 |
| Rwork | 0.211 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.062 | 0.335 |
| Number of reflections | 13374 | |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.6 | 7.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | 25 % PEG 4000, 0.1 M Tris-HCl, 0.2 M calcium chloride, pH 8.5, Microbatch, temperature 293K |
