2P5K
Crystal structure of the N-terminal domain of AhrC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.108, 32.900, 66.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.000 |
| R-factor | 0.139 |
| Rwork | 0.137 |
| R-free | 0.16000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f9n N-terminal domain of AhrC chain A |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.595 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.094 | 0.216 |
| Number of reflections | 26874 | |
| <I/σ(I)> | 12.8 | 2.8 |
| Completeness [%] | 89.4 | 70.6 |
| Redundancy | 4.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 24% PEG 8000, 0.1M sodium cacodylate, 0.1M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
