2P4Z

A Ferredoxin-like Metallo-beta-lactamase Superfamily Protein from Thermoanaerobacter tengcongensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	77.197, 138.310, 52.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.20118
Rwork	0.200
R-free	0.21855
Structure solution method	SIRAS
RMSD bond length	0.010
RMSD bond angle	1.278
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.078
Number of reflections	34056
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	24.5% Pentaerythritol propoxylate (5/4 PO/OH), 0.1M Bis-Tris, 0.08M Ammonium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K