2P4X
Crystal Structure of Atp12 from Paracoccus Denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-06 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.854, 50.632, 67.581 |
| Unit cell angles | 73.87, 74.19, 89.78 |
Refinement procedure
| Resolution | 48.490 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.490 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.656 |
| Number of reflections | 40173 | |
| <I/σ(I)> | 23.3 | 1.72 |
| Completeness [%] | 97.3 | 92.4 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | PEG 4000, Acetate, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
