2P4B
Crystal structure of E.coli RseB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9794 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.683, 197.681, 108.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.400 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.28300 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.067 | 0.369 |
Number of reflections | 39529 | |
<I/σ(I)> | 16 | |
Completeness [%] | 97.8 | 100 |
Redundancy | 5.5 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 6%(w/v) PEG 8000, 0.1M HEPES, 0.1M NaCl, 5%(v/v) MPD, 5%(w/v) guanidine-HCl, 3mM b-octylglucoside, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |