2P43
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.65A resolution: SE3-mono-1 crystal form with three se-met sites (M34, M51, M83) in vhh scaffold
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97955, 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.257, 54.435, 48.054 |
| Unit cell angles | 90.00, 108.45, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.16052 |
| Rwork | 0.158 |
| R-free | 0.20227 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.097 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.063 |
| Number of reflections | 23613 |
| Completeness [%] | 99.9 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 295 | HAMPTON RESEARCH INDEX SCREEN, SOLUTION #89: PEG 3350, SUCCINIC ACID, PH 7.0, VAPOR DIFFUSION, temperature 295K |
