2P3K
Crystal structure of Rhesus rotavirus VP8* at 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.887, 47.887, 129.932 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.560 |
| R-factor | 0.17336 |
| Rwork | 0.172 |
| R-free | 0.20009 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.171 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.087 | 0.115 |
| Number of reflections | 21759 | |
| <I/σ(I)> | 6.2 | 5.3 |
| Completeness [%] | 96.1 | 93.4 |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | 1.7M (NH4)2SO4, 2.4% v/v PEG 400, 0.1M PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
