2P3H
Crystal structure of the CorC_HlyC domain of a putative Corynebacterium glutamicum hemolysin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-23 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.91946 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.200, 51.214, 101.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.610 - 1.800 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.22800 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.428 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.064 | 0.393 |
Number of reflections | 13454 | |
<I/σ(I)> | 13.3 | |
Completeness [%] | 97.4 | 77.7 |
Redundancy | 7.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 291 | 2M (NH4)2SO4, 0.1M Cacodylate pH 6.5, 0.2M NaCl, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |