2P3H
Crystal structure of the CorC_HlyC domain of a putative Corynebacterium glutamicum hemolysin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.91946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.200, 51.214, 101.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.610 - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.428 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.064 | 0.393 |
| Number of reflections | 13454 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 97.4 | 77.7 |
| Redundancy | 7.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 291 | 2M (NH4)2SO4, 0.1M Cacodylate pH 6.5, 0.2M NaCl, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
