2P39

Crystal structure of human FGF23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97924
Spacegroup name	P 21 21 21
Unit cell lengths	38.811, 47.094, 84.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.500
Rwork	0.203
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p23
RMSD bond length	0.006
RMSD bond angle	1.374
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	25550
<I/σ(I)>	26.1
Completeness [%]	99.5	95.6
Redundancy	11.9	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	100 mM Tris-HCl, 1.0 M (NH4)2SO4, 10 mM [Co(NH3)6]Cl3, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K