2P38
Crystal Structure of Pyrococcus Abyssi Protein Homologue of Saccharomyces Cerevisiae NIP7P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-04 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.492, 90.284, 63.347 |
Unit cell angles | 90.00, 134.29, 90.00 |
Refinement procedure
Resolution | 31.670 - 1.800 |
R-factor | 0.21 |
Rwork | 0.207 |
R-free | 0.25700 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.710 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.670 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 33002 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 4.1 M NACL, 100 MM HEPES, PH 7.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, pH 7.20 |